|LOX-DB - LipOXygenases DataBase|
LOX-DB - Literature
Murine epidermal lipoxygenase (Aloxe) encodes a 12-lipoxygenase isoform.
Kinzig A., Fuerstenberger G., Mueller F., Vogel S., Mueller-Decker K., Mincheva A., Lichter P., Marks F., Krieg P.FEBS Lett. 402: 162-166 (1997)
Using a combination of conventional screening procedures and polymerase chain reaction cloning, we have isolated a cDNA encoding an epidermis-type 12-lipoxygenase (e12-lipoxygenase) from mouse epidermis. The open reading frame corresponds to a protein of 662 amino acids and was found to be 99.8% identical to the ORF of an epidermal lipoxygenase gene Aloxe, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4-8]. When expressed in human embryonic kidney cells the recombinant protein could be shown to synthesize 12(S)-HETE from arachidonic acid. By fluorescence in situ hybridization the e12-lipoxygenase gene was localized to chromosome band 11 B1-B3.