|LOX-DB - LipOXygenases DataBase|
LOX-DB - Literature
Three-dimensional structure of a purple lipoxygenase.
Skrzypczak-Jankun E., Bross R.A., Carroll R.T., Dunham W.R., Funk M.O. Jr.J. Am. Chem. Soc. 123:10814-10820 123: 10814-10820 (2001)
Polyunsaturated fatty acid metabolism is governed primarily by two enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure of the metastable product-oxidized purple form of soybean lipoxygenase-3 was determined at 2.0 A resolution. The data reveal that the chromophore corresponds to an iron-peroxide complex, a potential intermediate in the catalyzed reaction. A significant alteration of the iron site accompanies the formation of the complex. The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity.