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LOX-DB - Literature


Murine 12(R)-lipoxygenase: functional expression, genomic structure and chromosomal localization.

Krieg P , Siebert M , Kinzig A , Bettenhausen R , Marks F , Furstenberger G

FEBS Lett 446: 142-148 (1999)

Abstract:

A cDNA, recently cloned (by Krieg et al. (1998)) from mouse skin, was shown to encode a 12(R)-lipoxygenase. When expressed in HEK cells, the recombinant protein converted methyl arachidonate into the corresponding 12-HETE ester which was shown to be the R-enantiomer by chiral phase chromatography. Neither arachidonic acid nor linoleic acid were substrates for the recombinant protein. The structure of the 12(R)-lipoxygenase gene is unique among all animal lipoxygenases in that it is divided into 15 exons and 14 introns spanning approximately 12.5 kb. By interspecific backcross analysis, the 12(R)-lipoxygenase gene was localized to the central region of mouse chromosome 11.

LOX-DB entries related to this article: m-12r-lox