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Primary structure of soybean lipoxygenase-1.

Shibata D., Steczko J., Dixon J.E., Hermodson M., Yazdanparast R., Axelrod B.

J. Biol. Chem. 262: 10080-10085 (1987)

Abstract:

The primary structure of lipoxygenase-1 from soybeans has been determined. The results were deduced from the nucleotide sequence of the complete cDNA for isozyme lipoxygenase-1 and were confirmed by Edman sequencing of selected peptides obtained by CNBr cleavage and supported by carboxyl-terminal sequencing of the intact protein and by partial peptide analysis employing fast atom bombardment-mass spectroscopy. The enzyme has a molecular weight of 94,038 based on its content of 838 amino acids.

LOX-DB entries related to this article: gm-lox-1