Glycosciences.de database - bibliography entry

Bibliography entry #6158

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Reference Details

Taketa K; Fujii Y; Taga H

Characterization of E-PHA-reactive .alpha.-fetoprotein isoforms by two-dimensional lectin affinity electrophoresis

Published 1993 in Electrophoresis, 14: 1333-1337

PubMed: 7511099

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Abstract

Erythroagglutinating phytohemagglutinin (E-PHA)-dependent isoforms of human alpha-fetoprotein (AFP) from cord blood were analyzed for their carbohydrate structures by two-dimensional electrophoresis with E-PHA combined with extended agarose gel electrophoresis or with affinity electrophoresis with concanavalin A or Allomyrina dichtoma lectin. By means of neuraminidase and/or beta-galactosidase treatment, AFP-P2 was identified as alpha 2-->6 disialo-AFP, AFP-P3 as having biantennary structures with alpha 2-->6 monosialylated galactose of the Mannose (Man) alpha 1-->6 arm, AFP-P4 as having alpha 2-->6 monosialylated galactose of the Man alpha 1-->3 arm, and AFP-P5 as disialo-AFP with alpha 2-->3 sialylated galactose of the Man alpha 1-->6 antenna with the alpha 2-->6 sialylated galactose of the other antenna. Desialylated AFP with the terminal galactose of the Man alpha 1-->6 antenna with or without the galactose of the other arm also had a migration of AFP-P4, and other hydrolytic intermediates without the terminal galactose of the Man alpha 1-->6 arm with and without the galactose of the other antenna had mobilities of AFP-P3s and AFP-P3, respectively. Thus, the present system of two-dimensional lectin affinity electrophoreses would provide a model for the determination of the sugar chain structure of glycoproteins.

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Glycan structures: 11

1 - 5 of 11 carbohydrate structure entries

Structure entry #268:

a-L-Fucp-(1-6)+ | b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)+ b-D-GlcpNAc-(1-4)-Asn | | b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)+ | a-D-Neup5Ac-(2-6)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)+

1H: 1

: 18

Taxons: 3

Structure entry #269:

a-L-Fucp-(1-6)+ | a-D-Neup5Ac-(2-6)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)+ b-D-GlcpNAc-(1-4)-Asn | | b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)+ | a-D-Neup5Ac-(2-6)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)+

1H: 1

: 54

Taxons: 5

Structure entry #270:

a-L-Fucp-(1-6)+ | a-D-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)+ b-D-GlcpNAc-(1-4)-Asn | | b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)+ | a-D-Neup5Ac-(2-6)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)+

1H: 1

: 4

Taxons: 2

Structure entry #2387:

a-L-Fucp-(1-6)+ | b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)+ b-D-GlcpNAc-(1-4)-Asn | | b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)+ | a-D-Neup5Ac-(2-6)-b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)+

: 1

: 10

Taxons: 1

Structure entry #3657:

a-L-Fucp-(1-6)+ | b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-6)+ b-D-GlcpNAc-(1-4)-Asn | | b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)+ | b-D-Galp-(1-4)-b-D-GlcpNAc-(1-2)-a-D-Manp-(1-3)+