Title: Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritriose (Form II)
Description: Sugar ABC transporter, periplasmic sugar-binding protein
Keywords: Conformational dynamics, substrate-binding protein, Induced-fit mechanism, Two-step ligand binding, Venus Fly-trap mechanism, SUGAR BINDING PROTEIN
Source: Thermus thermophilus HB8
Experimental method: X-RAY DIFFRACTION
Resolution: 2.8Å
Release date: 2020-09-16
Entity 1: Sugar ABC transporter, periplasmic sugar-binding protein
Strand: A,B,C
Type: polymer
Polymer type: polypeptide(L)
Source: Thermus thermophilus HB8 (Taxonomy ID:
300852)
Expression system: Escherichia coli BL21(DE3)
Source gene: TTHB082
Entity 2: beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose
Type: branched
Entity 3: CHLORIDE ION
Type: non-polymer
Entity 4: SULFATE ION
Type: non-polymer
Entity 5: 1,2-ETHANEDIOL
Type: non-polymer
Entity 6: DI(HYDROXYETHYL)ETHER
Type: non-polymer
Entity 7: SULFITE ION
Type: non-polymer
Entity 8: water
Type: water
Structure entry #27365 [3 instances]
b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp