Title: Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open-liganded state bound to laminaribiose
Description: Sugar ABC transporter, periplasmic sugar-binding protein
Keywords: Conformational dynamics, substrate-binding protein, Induced-fit mechanism, Two-step ligand binding, Venus Fly-trap mechanism, SUGAR BINDING PROTEIN
Source: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Experimental method: X-RAY DIFFRACTION
Resolution: 1.85Å
Release date: 2020-10-21
Entity 1: Sugar ABC transporter, periplasmic sugar-binding protein
Strand: A,B
Type: polymer
Polymer type: polypeptide(L)
Source: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (Taxonomy ID:
300852)
Expression system: Escherichia coli BL21(DE3)
Source gene: TTHB082
Entity 2: beta-D-glucopyranose-(1-3)-beta-D-glucopyranose
Type: branched
Synonyms: beta-laminaribiose
Entity 3: CHLORIDE ION
Type: non-polymer
Entity 4: ACETATE ION
Type: non-polymer
Entity 5: SULFATE ION
Type: non-polymer
Entity 6: 1,2-ETHANEDIOL
Type: non-polymer
Entity 7: S-1,2-PROPANEDIOL
Type: non-polymer
Entity 8: DI(HYDROXYETHYL)ETHER
Type: non-polymer
Entity 9: water
Type: water